In vivo assays with natural promoters

Efficacy of Eugenol against a Salmonella enterica serovar Enteritidis experimental infection in commercial layers in production

2008 J. Appl. Poult. Res. 17:376-382
G. Ordoñez², N. Llopis¹, P. Peñalver¹

1 Lidervet S.L., Tarragona, Spain
2 CESAC, Centre de Sanitat Avícola de Catalunya i Aragó, Reus, Spain


An experimental study (15-week-old ISA Brown pullets) was conducted to establish the efficacy of the essential oil of Eugenia caryophyllata against Salmonella enterica serovar Enteritidis. The trial was composed of 4 groups. Pullets in groups 3 and 4 were fed with a commercial compound feed, and pullets in groups 1 and 2 were fed with the same feed plus the aromatic product at the dose of 250 g/Tm. At 19 weeks old, the pullets in groups 1 and 3 were infected individually with an inoculum of 3.2±0.8x10 7 cfu of Salmonella enterica serovar Enteritidis/pullet. During the postinoculation period, samples of feces and eggs were cultured, and pullets were killed 30 days postinoculation. The aromatic product containing eugenol seems to aid in the cleaning of the intestinal and systemic infections, and it also plays an important role in the control of Salmonella cross contamination in eggs.

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